Structural Analysis of the Lipopeptide Produced by the Bacillus subtilis Mutant R2-104 with Mutagenesis
作者： Yong Meng , Wei Zhao , Jia You , Hong-Ze Gang , Jin-Feng Liu
DOI: 10.1007/S12010-016-2044-5
关键词: Surfactin 、 Mutant 、 Bacillus subtilis 、 Stereochemistry 、 Lipopeptide 、 Strain (chemistry) 、 Mutagenesis (molecular biology technique) 、 Combined method 、 Microbiology 、 Yield (chemistry) 、 Chemistry
摘要: The lipopeptide and its homologues are a kind of the five major biosurfactants with prominent interfacial biological activities. A suite mutagenesis method was adopted to expose wild lipopeptide-producing strain Bacillus subtilis HSO121 improve yield, stable mutant named R2-104 2.0-fold production obtained. Compared that HSO121, produced by showed similar surface activity, but course profiles during cultivation were different, peak yield 500 mg at about 9 h R2-104, 400 mg 5 h HSO121. constituent abundance also different from Combined methods ESI-MS, GC-MS MS-MS applied for structural characterization homologues, it lipopeptides attributed surfactin family constituents. dominant anteiso C15-surfactin relative content 43.8 %, while one HSO121was iso C14-surfactin 33.1 %.
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