Investigations of the Biological Effects of Airborne and Inhalable Substances by Cell-Based In Vitro Methods: Fundamental Improvements to the ALI Concept

摘要: The state of the art for cell-based in vitro investigations airborne and inhalable material is “air-liquid interface” (ALI) technology. Cell lines, primary cells, complex 3D models, or precision-cut lung slices (PCLS) are used to represent skin by way an barrier model. These models have been applied toxicity pharmacological testing. However, contrasting with a clear demand alternative methods, there still no widely accepted procedure testing substances. In light this, analysis was undertaken common drawbacks current approaches. Hence, pivotal improvements aimed at were cellular exposure environment, overall performance applicability, operability online during routine setup. It resulted improved device (P.R.I.T. ExpoCube) based on “all-in-one-plate” concept including all phases experiment (cell culture, exposure, read-out) experimental groups (two test gas groups, controls) one single commercial multiwell plate. Verification demonstrated first series using reference substances (formaldehyde, ozone, clean air). resulting ALI enables application mixtures under highly effective conditions utilization.