Prognostic significance of K-ras, p53, bcl-2, PCNA, CD34 in radically resected non-small cell lung cancers.

摘要: Abstract The aim of this study was to investigate the prognostic significance a panel biological parameters in patients with radically resected non-small cell lung cancers (NSCLC). 269 cases pathological stage I-IIIA NSCLC were retrospectively analysed. Immunohistochemistry performed detect protein expression p53, bcl-2, proliferating nuclear antigen (PCNA) and CD34. Polymerase chain reaction (PCR)/direct nucleotide sequencing method used mutations K- ras (codons 12, 13, 61, exons 1-2). Kaplan–Meier estimates survival calculated for clinical variables using Cox model multivariate analysis. Histological subtype pathologic tumour extension (pT) most powerful clinical–pathological factors ( P =0.030 =0.031, respectively), whereas among parameters, p53 overexpression =0.032) mutation =0.078) had negative role, as demonstrated by Conversely, PCNA CD34 not correlated survival. Statistically significant associations between squamous carcinoma (SCC) subtype, bcl-2 SCC expression, observed. In conclusion, some characteristics such status may provide useful information patients, addition classical clinico-pathological parameters. However, further studies are needed clarify value adopting factor into practice.