Expression of dominant-negative ErbB2 in the mammary gland of transgenic mice reveals a role in lobuloalveolar development and lactation.
作者： Frank E Jones , David F Stern
关键词: Phenotype 、 Receptor tyrosine kinase 、 Internal medicine 、 Mammary gland 、 Immunohistochemistry 、 Genetically modified mouse 、 Endocrinology 、 Cell surface receptor 、 Lactation 、 Biology 、 Signal transduction
摘要: Overexpression of the receptor tyrosine kinase ErbB2/HER2/Neu (ErbB2) occurs in 15-40% human breast cancers. To determine function ErbB2 signaling during normal mouse mammary gland development, we expressed a carboxyl-terminal truncated dominant negative allele (ErbB2deltaIC) developing gland. Despite ErbB2deltaIC expression within glands pubescent virgin and pregnant mice, phenotype was not observed until late pregnancy. At 1 day post-partum, lactationally active, distended lobuloalveoli failed to form. This exaggerated multiparous females expressing ErbB2deltaIC. Immunohistochemical staining for revealed concordance between high levels protein absence lactational products lumens stained lobuloalveoli. These results demonstrate that is required proper development lactation at parturition.
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