Identification of an 85 kDa phosphoprotein as an immunodominant protein specific for human herpesvirus 7-infected cells
作者： M. P. Fiorilli , E. Avitabile , G. Campadelli-Fiume , L. Foa-Tomasi
DOI: 10.1099/0022-1317-77-3-511
关键词: Epitope 、 Western blot 、 Biology 、 Phosphorylation 、 Molecular mass 、 Phosphoprotein 、 Human herpesvirus 6 、 Antibody 、 Molecular biology 、 Virology 、 Antigen
摘要: The reactivity of human cord blood sera was directed most frequently in Western blot assays to a protein with an apparent molecular mass 85 kDa that belongs the p85 complex, family antigenically related proteins identified previously our laboratory aid two MAbs. We show is phosphorylated. As antibodies present were part carrying cross-reactive epitopes between herpesvirus 6 (HHV-6) and 7 (HHV-7), it remarkable phosphoprotein maintained after preabsorption HHV-6 antigen, but abolished HHV-7 antigen. Therefore, may be considered major determinant immune response HHV-7, discriminating from infection.
sgmjournals.org参考文章(16)
M Yamamoto, J B Black, J A Stewart, C Lopez, P E Pellett, Identification of a nucleocapsid protein as a specific serological marker of human herpesvirus 6 infection. Journal of Clinical Microbiology. ,vol. 28, pp. 1957- 1962 ,(1990) , 10.1128/JCM.28.9.1957-1962.1990
L S Wyatt, N Frenkel, Human herpesvirus 7 is a constitutive inhabitant of adult human saliva. Journal of Virology. ,vol. 66, pp. 3206- 3209 ,(1992) , 10.1128/JVI.66.5.3206-3209.1992
L S Wyatt, W J Rodriguez, N Balachandran, N Frenkel, Human herpesvirus 7: antigenic properties and prevalence in children and adults. Journal of Virology. ,vol. 65, pp. 6260- 6265 ,(1991) , 10.1128/JVI.65.11.6260-6265.1991
L Foà-Tomasi, A Boscaro, S di Gaeta, G Campadelli-Fiume, Monoclonal antibodies to gp100 inhibit penetration of human herpesvirus 6 and polykaryocyte formation in susceptible cells Journal of Virology. ,vol. 65, pp. 4124- 4129 ,(1991) , 10.1128/JVI.65.8.4124-4129.1991
L. Foa-Tomasi, E. Avitabile, L. Ke, G. Campadelli-Fiume, Polyvalent and monoclonal antibodies identify major immunogenic proteins specific for human herpesvirus 7-infected cells and have weak cross-reactivity with human herpesvirus 6. Journal of General Virology. ,vol. 75, pp. 2719- 2727 ,(1994) , 10.1099/0022-1317-75-10-2719
Jodi B. Black, Naoki Inoue, Kathleen Kite-Powell, Sherif Zaki, Philip E. Pellett, Frequent isolation of human herpesvirus 7 from saliva. Virus Research. ,vol. 29, pp. 91- 98 ,(1993) , 10.1016/0168-1702(93)90128-A
Kohji Ueda, Koichi Kusuhara, Kenji Okada, Chiaki Miyazaki, Yasufumi Hidaka, Ken Tokugawa, Koichi Yamanishi, Primary human herpesvirus 7 infection and exanthema subitum. Pediatric Infectious Disease Journal. ,vol. 13, pp. 167- ,(1994) , 10.1097/00006454-199402000-00027
Tetsushi Yoshikawa, Yoshizo Asano, Ikuko Kobayashi, Toshihiko Nakashima, Takehiko Yazaki, Sadao Suga, Takao Ozaki, Linda S. Wyatt, Niza Frenkel, Seroepidemiology of human herpesvirus 7 in healthy children and adults in Japan. Journal of Medical Virology. ,vol. 41, pp. 319- 323 ,(1993) , 10.1002/JMV.1890410412
G. Jahn, B.-C. Scholl, B. Traupe, B. Fleckenstein, The Two Major Structural Phosphoproteins (pp65 and pp150) of Human Cytomegalovirus and Their Antigenic Properties Journal of General Virology. ,vol. 68, pp. 1327- 1337 ,(1987) , 10.1099/0022-1317-68-5-1327
Philip E. Pellett, Demetrio Sánchez-Martı́nez, Geraldina Dominguez, Jodi B. Black, Elizabeth Anton, Cynthia Greenamoyer, Timothy R. Dambaugh, A Strongly Immunoreactive Virion Protein of Human Herpesvirus 6 Variant B Strain Z29: Identification and Characterization of the Gene and Mapping of a Variant-Specific Monoclonal Antibody Reactive Epitope Virology. ,vol. 195, pp. 521- 531 ,(1993) , 10.1006/VIRO.1993.1403