Atomic force microscopy as a tool for the investigation of living cells.

摘要: Summary. Atomic force microscopy is a valuable and useful tool for the imaging investigation of living cells in their natural environment at high resolution. Procedures applied to cell preparation before measurements should be adapted individually different kinds desired measurement technique. Different ways immobilization, such as chemical fixation on surface, entrapment pores membrane, or growing them directly glass cover slips plastic substrates, result distortion appearance artifacts atomic images. Cell allows multiple use samples storage prolonged period; it also increases resolution imaging. modes are used analysis cells. The contact mode best because resolution, but usually based following: (i) image formation low interaction force, (ii) scanning speed, (iii) usage “soft,” cantilevers. tapping behave like very solid material, destructive shear forces minimized, liquid difficult. spectroscopy measuring mechanical properties cells; however, obtained results strongly depend method. In this paper, application 3 including contact, tapping, described. possibilities measurements, imaging, determination provided. applicability diagnostics other biomedical purposes discussed.