Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system.
作者： Qin Yang , Xianzhe Shi , Yuan Wang , Wenzhao Wang , Hongbin He
关键词: Hydrophilic interaction chromatography 、 Orthogonal signal correction 、 Fully automatic 、 Lung cancer 、 Column switching 、 Urinary system 、 Potential biomarkers 、 Chromatography 、 Chemistry 、 Metabolite
摘要: Lung cancer is one of the most common and lethal cancers in world. In this study, a home-devised hydrophilic interaction chromatography/RPLC-MS (HILIC/RPLC-MS) system was developed to study urinary metabonomics lung patients. This combined orthogonal selectivity HILIC RPLC could chromatographically reveal more comprehensive information metabolites. Within total analysis time 50 min, we detected 577 polar metabolite ions on first column 261 apolar ones second column. addition, an signal correction partial least-squares discriminant model constructed characterize differences between health cases. Eleven potential biomarkers, ten from RP column, were identified all these biomarkers found upregulated Overall, results indicated that HILIC/RPLC-MS promising tool for metabonomic studies revealing highly complex samples.
irgrid.ac.cn参考文章(50)
Yoshiharu Shimomura, Yuko Yamamoto, Gustavo Bajotto, Juichi Sato, Taro Murakami, Noriko Shimomura, Hisamine Kobayashi, Kazunori Mawatari, Nutraceutical Effects of Branched-Chain Amino Acids on Skeletal Muscle Journal of Nutrition. ,vol. 136, ,(2006) , 10.1093/JN/136.2.529S
Kazuki Akira, Shigenori Masu, Misako Imachi, Hidemichi Mitome, Miho Hashimoto, Takao Hashimoto, 1H NMR-based metabonomic analysis of urine from young spontaneously hypertensive rats. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 46, pp. 550- 556 ,(2007) , 10.1016/J.JPBA.2007.11.017
D.R. Ekman, Q. Teng, K.M. Jensen, D. Martinovic, D.L. Villeneuve, G.T. Ankley, T.W. Collette, NMR analysis of male fathead minnow urinary metabolites: a potential approach for studying impacts of chemical exposures. Aquatic Toxicology. ,vol. 85, pp. 104- 112 ,(2007) , 10.1016/J.AQUATOX.2007.08.005
Michael Phillips, Nasser Altorki, John H.M. Austin, Robert B. Cameron, Renee N. Cataneo, Robert Kloss, Roger A. Maxfield, Muhammad I. Munawar, Harvey I. Pass, Asif Rashid, William N. Rom, Peter Schmitt, James Wai, Detection of lung cancer using weighted digital analysis of breath biomarkers Clinica Chimica Acta. ,vol. 393, pp. 76- 84 ,(2008) , 10.1016/J.CCA.2008.02.021
Gianfranco Peluso, Raffaella Nicolai, Emilia Reda, Paola Benatti, Alfonso Barbarisi, Menotti Calvani, Cancer and anticancer therapy‐induced modifications on metabolism mediated by carnitine system Journal of Cellular Physiology. ,vol. 182, pp. 339- 350 ,(2000) , 10.1002/(SICI)1097-4652(200003)182:3<339::AID-JCP4>3.0.CO;2-B
Yousuke Fukui, Masao Kato, Yohji Inoue, Akio Matsubara, Kohji Itoh, A metabonomic approach identifies human urinary phenylacetylglutamine as a novel marker of interstitial cystitis Journal of Chromatography B. ,vol. 877, pp. 3806- 3812 ,(2009) , 10.1016/J.JCHROMB.2009.09.025
Stephen J. Bruce, Pär Jonsson, Henrik Antti, Olivier Cloarec, Johan Trygg, Stefan L. Marklund, Thomas Moritz, Evaluation of a protocol for metabolic profiling studies on human blood plasma by combined ultra-performance liquid chromatography/mass spectrometry: From extraction to data analysis. Analytical Biochemistry. ,vol. 372, pp. 237- 249 ,(2008) , 10.1016/J.AB.2007.09.037
Alex Soltermann, Reto Ossola, Sandra Kilgus-Hawelski, Arnold von Eckardstein, Tobias Suter, Ruedi Aebersold, Holger Moch, N-glycoprotein profiling of lung adenocarcinoma pleural effusions by shotgun proteomics. Cancer. ,vol. 114, pp. 124- 133 ,(2008) , 10.1002/CNCR.23349
John P. Shockcor, Steve E. Unger, Ian D. Wilson, Peta J. D. Foxall, Jeremy K. Nicholson, John C. Lindon, Combined HPLC, NMR spectroscopy, and ion-trap mass spectrometry with application to the detection and characterization of xenobiotic and endogenous metabolites in human urine. Analytical Chemistry. ,vol. 68, pp. 4431- 4435 ,(1996) , 10.1021/AC9606463
Helen G. Gika, Georgios A. Theodoridis, Julia E. Wingate, Ian D. Wilson, Within-Day Reproducibility of an HPLC−MS-Based Method for Metabonomic Analysis: Application to Human Urine Journal of Proteome Research. ,vol. 6, pp. 3291- 3303 ,(2007) , 10.1021/PR070183P