Inhibition of growth factor-induced differentiation of PC12 cells by microinjection of antibody to ras p21
作者： Nabil Hagag , Simon Halegoua , Michael Viola
DOI: 10.1038/319680A0
关键词: Cell growth 、 Growth factor 、 Cell biology 、 Neurite 、 Internal medicine 、 Cellular differentiation 、 Cell culture 、 Endocrinology 、 Nerve growth factor 、 Cell division 、 Biology 、 Microinjection
摘要: The protein products (p21) of the ras cellular proto-oncogenes are thought to transduce membrane signals necessary for induction cell division. However, there is uncertainty as precise role p21 in mediating ligand-membrane receptor leading differentiation. Treatment rat phaeochromocytoma cells (PC12) with nerve growth factor (NGF) results a number phenotypic characteristics sympathetic neurones, including cessation division and outgrowth neuronal processes (neurites). Here we report that microinjection antibody into PC12 inhibited neurite formation resulted temporary regression partially extended neurites, an effect which was observed up 36 h after initiation NGF treatment. Neurite induced by cyclic AMP unaffected injection anti-p21 antibody. These indicate involved phase NGF-induced has hormone-mediated responses distinct from proliferation.
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