MCP-1 expression in CNS-1 astrocytoma cells: implications for macrophage infiltration into tumors in vivo.

摘要: Gliomas are among the most resistant tumors to conventional anti-tumor therapy, and typified by their highly infiltrative nature ill-defined borders. Macrophages constitute a major proportion of tumor cell mass in both primary human gliomas as shown here, CNS-1 glioma model. The objective this study was identify tumor-cell-derived chemotactic factor(s) which participate macrophage recruitment into vivo. This demonstrates constitutive expression monocyte chemoattractant protein-1 (MCP-1), potent chemoattractant, rat astrocytoma line CNS-1. Characterization cytokine cells vitro revealed TGF-β but not other proinflammatory cytokines. However, numerous cytokines were detected vivo including Ltβ, IL-1α, IL-1β, TNF-α, TNF-β, IL-10, IFN-γ. Attenuation MCP-1 release from using an anti-sense approach no significant alterations infiltration vivo, suggesting redundancy signal(s) involved recruitment. Depletion peripheral macrophages liposome-encapsulated clodronate differences growth or degree These results indicate that produce factors likely Whether confers advantage for remains be determined.