NR2F1-AS1 regulated miR-423-5p/SOX12 to promote proliferation and invasion of papillary thyroid carcinoma.
作者： Chuanjia Yang , Zhen Liu , Xiaoying Chang , Weixue Xu , Jian Gong
DOI: 10.1002/JCB.29435
关键词: Thyroid carcinoma 、 Cancer research 、 Gene 、 Western blot 、 Biology 、 Antisense RNA 、 Gene expression profiling 、 Cell growth 、 Rna immunoprecipitation 、 Luciferase reporter
摘要: Papillary thyroid carcinoma (PTC) is an aggressive histological subtype of (THCA), whose occurrence rate high. The participation long noncoding RNAs in the pathologies cancers has attracted significant attention during past decades. purpose current study to investigate role NR2F1 antisense RNA 1 (NR2F1-AS1) PTC. expression THCA samples was analyzed by bioinformatics tool gene profiling interactive analysis. Levels NR2F1-AS1, microRNA-423-5p (miR-423-5p), and SRY-box 12 (SOX12) were evaluated a quantitative reverse transcription-polymerase chain reaction Western blot. impact NR2F1-AS1 on PTC cell proliferation invasion assessed Cell Counting Kit-8, EdU, Transwell assays. interactions among miR-423-5p, SOX12 determined immunoprecipitation luciferase reporter Consequently, we found that levels elevated PTC, whereas miR-423-5p downregulated cells. Functionally, silence led reduced Mechanistically, interacted with induce In conclusion, present firstly stated regulated miR-423-5p/SOX12 promote indicating as potential novel target for molecular-targeted therapy
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