T cell subset distributions following primary and secondary implantation at subcutaneous biomaterial implant sites.

摘要: Synthetic biomaterials are considered to be nonimmunogenic. Therefore, the role that adaptive immunity may play in host response implanted synthetic has not been extensively studied. Cardinal features of include specificity and T cell responses, which greater more effective with upregulation activation receptors upon rechallenge. We compared primary secondary vivo three biomaterials: Elasthane 80A, silicone rubber, polyethylene terephthalate using a cage implant model Sprague Dawley rats. The biomedical polymers were subcutaneously cages for 14 days. Following explantation 2 week healing period, rats containing an additional weeks. cellular exudates within analyzed 4, 7, days post implantation by flow cytometry following types: cells (inclusive CD8(+), CD4(+), CD4(+)/CD25(+) subsets), B cells, granulocytes, macrophages. At day implantation, there was increase macrophages when all groups inclusive empty control. However, CD4(+)/CD8(+) ratios, percentage CD4(+)CD25(+) macrophage surface adhesion/fusion did vary significantly implantation. Despite quantitative biomaterial exposure, subset distribution change, indicating nonspecific recruitment rather than immune response.