Identification of a novel type of alternative splicing of a tyrosine kinase receptor. Juxtamembrane deletion of the c-met protein kinase C serine phosphorylation regulatory site.

摘要: We have detected a novel type of structural variant the tyrosine kinase receptor for c-met, also known as hepatocyte growth factor receptor, in mouse tissues. The cDNA transcript c-met lacks 141 base pairs, which predicts an in-frame deletion 47 amino acids juxtamembrane region cytoplasmic domain. Sequence analysis genomic DNA containing locus revealed that absence discrete exon is responsible this 141-base pair and alternative splicing leads to production two forms transcript. These are designated c-metsm (for small) c-metlg large) distinguish or presence segment, respectively. present adult tissues including kidney, liver, brain well 9-10-day-old embryos. In all cases, expression was lower than normal transcript, c-metlg. An antiserum against c-Met protein immunoprecipitated corresponding approximately 152 145 kDa from whole kidney lysate under reducing conditions. size difference 7 between these isoforms corresponds predicted acids. shorter its isoform variety suggests physiological role. deleted domain contains sequence motif (S985ARS) C phosphorylation has recently been shown play key role down-regulation activity. identification isoform, c-metsm, demonstrates can achieve additional diversity by at regulatory site