3D quantitative analyses of angiogenic sprout growth dynamics

摘要: Background: Zebrafish intersegmental vessel (ISV) growth is widely used to study angiogenesis and screen drugs toxins that perturb angiogenesis. Most current ISV assays observe the presence or absence of ISVs perturbation morphology but do not measure dynamics. We have developed a four-dimensional (4D, space plus time) quantitative analysis angiogenic sprout dynamics for characterization both normal perturbed growth. Results: tracked positions base tip each in 4D. Despite immobilization, zebrafish embryos translocated globally non-uniformly during development. displacement angle between dorsal aorta correct rotation From corrected cell coordinates, we computed average trajectories. fitted quadratic curve trajectories produce canonical trajectory experimental group, arsenic treated untreated. trajectories, curvature, directed migration speed directionality. Canonical from (arsenic exposed) untreated groups differed aorta. Conclusions: 4D dynamics: (1) Allows assessment perturbation, (2) provides critical inputs computational models Developmental Dynamics 242:518–526, 2013. V C 2013 Wiley Periodicals, Inc.