Response-Predictive Gene Expression Profiling of Glioma Progenitor Cells In Vitro
作者： Sylvia Moeckel , Katharina Meyer , Petra Leukel , Fabian Heudorfer , Corinna Seliger
DOI: 10.1371/JOURNAL.PONE.0108632
关键词:
摘要: Background High-grade gliomas are amongst the most deadly human tumors. Treatment results disappointing. Still, in several trials around 20% of patients respond to therapy. To date, diagnostic strategies identify that will profit from a specific therapy do not exist. Methods In this study, we used serum-free short-term treated vitro cell cultures predict treatment response vitro. This approach allowed us (a) enrich specimens for brain tumor initiating cells and (b) confront with therapeutic agent before expression profiling. Results As proof principle analyzed gene 18 high-grade enhanced (BTIC) after tyrosine kinase inhibitor Sunitinib. Profiles progenitor therapy-induced impairment proliferation vitro. Conclusion For Sunitinib dataset, revealed additional predictive information comparison evaluation classical signaling analysis.
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