K-ras and p53 Gene Mutations in Pancreatic Cancer: Ductal and Nonductal Tumors Progress through Different Genetic Lesions

摘要: We studied K-ras and p53 gene mutations in a panel of 57 primary pancreatic cancers including ductal nonductal tumors. DNAs were obtained from formalin-fixed, paraffin-embedded material. Target sequences amplified by polymerase chain reaction analyzed denaturing gradient gel electrophoresis sequencing. Both genes frequently mutated (25 35, 71.4%; 18 51.4%, respectively). confined to the second position codon 12 where base transitions transversions equally observed. changes mainly missense mutations. Transitions found with prevalence G:C-->A:T among transitions. No alterations present 6 exocrine tumors (with one exception) endocrine analyzed. Our results indicate that can cooperate establishment cancers, whereas other genetic events have be Moreover, may represent an early event tumorigenesis, as suggested both high mutation frequency presence low-grade On contrary, seem required for malignancy progression lower higher grades.