Gene amplification in malignant human gliomas: clinical and histopathologic aspects.

摘要: Gene amplification occurs in 45-50% of malignant human gliomas (MHG). In the present study, 64 genetically characterized were evaluated to determine if tumors with epidermal growth factor receptor (EGFR), N- myc , c- or gli genes had distinctive histopathologic features. There was no significant difference age (p = 0.10) gender 0.78) between patients whose contained amplified and those did not exhibit this characteristic. Although their survived slightly longer than detectable gene amplification, these differences statistically (p=0.21). The 28 included 24/48 (50%) glioblastoma multiforme, 2/6 (33%) anaplastic astrocytomas 2/5 (40%) gliosarcomas. No seen one oligodendroglioma, three mixed giant cell multiforme. Necrosis endothelial proliferation equally prevalent among without amplification. Comparison characteristic revealed similar distributions most morphologic cells types. prominent perivascular lymphocytic infiltrates more frequent association borderline significance statistically. situ hybridization using an EGFR mRNA probe showed intense labeling different neoplastic types including fibrillary protoplasmic astrocytes, gemistocytes, cells, multinucleated cells. Non-neoplastic such as hyperplastic endothelium within express mRNA. These studies demonstrate that (a) quite morphology same tumor can contain genetic alteration; (b) identical histological appearance may have arisen evolved by molecular mechanisms; (c) analyses are necessary evaluate MHG since cannot be accurately predicted clinical criteria used study.