Characterization of the pleiotropic roles of Sonic Hedgehog during retinal regeneration in adult zebrafish.

摘要: In contrast to the mammalian retina, zebrafish retina possesses ability regenerate. This is primarily accomplished through Muller glial cells, which, upon damage, re-enter cell cycle form retinal progenitors. The progenitors continue proliferate as they migrate area of damage and ultimately differentiate into new neurons. purpose this study was characterize expression function Sonic Hedgehog (Shh) during regeneration adult retina. Expression profiling Shh pathway genes showed a significant upregulation associated with stages progenitor proliferation neuronal differentiation. Activation signaling early using intraocular injections recombinant human SHH (SHH-N) resulted in increased gliosis, proliferation, neuroprotection damaged Continued activation greater number differentiated amacrine ganglion cells fully regenerated Conversely, inhibition cyclopamine decreased fewer cells. These data suggest that plays pleiotropic roles differentiation regeneration.