A Disintegrin and Metalloproteinase 10 Regulates Antibody Production and Maintenance of Lymphoid Architecture

摘要: A disintegrin and metalloproteinase 10 (ADAM10) is a zinc-dependent proteinase related to matrix metalloproteinases. ADAM10 has emerged as key regulator of cellular processes by cleaving shedding extracellular domains multiple transmembrane receptors ligands. We have developed B cell-specific ADAM10-deficient mice (ADAM10B−/−). In this study, we show that levels are significantly enhanced on germinal center cells. Moreover, ADAM10B−/− had severely diminished primary secondary responses after T-dependent immunization. displayed impaired formation, fewer follicular Th cells, decreased dendritic cell networks, altered chemokine expression in draining lymph nodes (LNs). Interestingly, when spleen LN structures from immunized were analyzed for T localization, tissues structure was aberrant mice. Importantly, cells stimulated vitro, they produced comparable Ab wild type This result demonstrates the defects humoral vivo inadequate activation, likely because decrease changes structure. Thus, essential maintenance lymphoid Ag challenge.