Cytoglobin has bimodal: tumour suppressor and oncogene functions in lung cancer cell lines

摘要: Cytoglobin (CYGB) is frequently downregulated in many types of human malignancies, and its exogenous overexpression reduces proliferation cancer cells. Despite implied tumour suppressor (TSG) functions, exact role carcinogenesis remains unclear as CYGB upregulation also associated with hypoxia aggressiveness. In this study, we explore the TSG CYGB, influence on phenotype cancerous cells under stress conditions clinical significance expression promoter methylation non-small cell lung (NSCLC). DNA methylation-dependent silencing demonstrated both samples lines. was more methylated adenocarcinomas (P = 1.4 × 10(-4)). Demethylation by 5'-azadeoxycytidine partially restored Interestingly, trichostatin A triggered lines downregulation non-tumourigenic ones. mRNA NSCLC surgical specimens correlated that HIF1α VEGFa < 1 Overexpression reduced migration, invasion anchorage-independent growth. Moreover, impaired proliferation, but only adenocarcinoma line (H358). Upon hydrogen peroxide treatment, protected viability, migratory potential anchorage independence attenuating oxidative injury. hypoxia, decreased augmented migration a cell-type-specific manner. conclusion, revealed properties normoxia promoted tumourigenic exposed to stress, suggesting bimodal function tumourigenesis, depending type microenvironmental conditions.