Cytogenetic analysis of paediatric astrocytoma using comparative genomic hybridisation and fluorescence in-situ hybridisation

摘要: Little is known about the cytogenetic and molecular genetic events that lead to formation of paediatric astrocytoma. We have analysed 57 astrocytoma (WHO grades I–IV) using comparative genomic hybridisation in order identify common regions abnormality. Large copy number alterations were infrequent with 71% tumours demonstrating no imbalance. Furthermore, most frequent aberrations (including gain 6q, 2q, 7q, loss 16 12q) occurred only a subset cases. High-copy amplification was seen five at 12 different regions. The presence significantly associated increasing grade malignancy, 12q high-copy poor outcome patients malignant (P = 0.0039 0.0085, respectively). FISH analysis confirmed 1p36 identified by CGH. There evidence EGFR, CDK4, MET, CDK6, c-myc, or MDM2.