K-ras and p16INK4Aalterations in sputum of NSCLC patients and in heavy asymptomatic chronic smokers

摘要: Abstract NSCLC rates among the most frequent and lethal neoplasm world-wide a significant decrease in morbidity mortality relies only upon effective early diagnostic strategies. We investigated K-ras mutations p16 INK4A hypermethylation tumor tissue sputum of 50 patients with correlated them cytology staging, grading location, to ascertain, sputum, their potential impact. The same genetic/epigenetic abnormalities cytological features were also evaluated from 100 chronic heavy smokers. Genetic analysis identified molecular 64% tumors (14/50 24/50 hypermethylation) 48% (11/50 16/50 hypermethylation). In mostly mutually exclusive, being found 3 cases only. detected abnormal tumors. Molecular changes had detection similar (42%) but cyto-molecular combination increased yield up 60%. Interestingly, rate genetic at stage (T1) was not significantly different that more advanced (T2–T4). fact point frequently recognised while inactivation prevailed ( P =0.0063). As expected, findings =0.004). No correlation between location (central versus peripheral) changes. hypermethylation, mutations, documented sporadic asymptomatic smokers (4%) where it uncoupled abnormalities. conclusion strategy adopted this study able detect majority order be proposed as tool, panel needs tested prospective studies adequate follow-up.