Kluyveromyces marxianus CDBB-L-278: A wild inulinase hyperproducing strain

摘要: Abstract Kluyveromyces marxianus CDBB-L-278 is an inulinase hyperproducing strain. It was able to grow in a medium containing inulin as the unique carbon source presence of 2-deoxyglucose. produced up 3.3 times activity control strain K. NCYC-1429 medium, and 3.6 with glycerol sole source. Although produce 2-deoxyglucose, it demonstrated that not de-repressed since enzyme production reduced when concentration glucose or fructose increased medium. Since without inducer, can be considered partially constitutive well NCYC-1429. The from characterized. had higher affinity for than sucrose. Temperature pH profiles were different both these two substrates. stable high temperatures, half-life 180 min at 50°C.