Cellular Sequences Related to Three New onc Genes of Avian Sarcoma Virus (fps, yes, and ros) and Their Expression in Normal and Transformed Cells

摘要: Two onc genes of avian sarcoma viruses unrelated to the src gene have recently been identified: fps Fujinami virus/PRCII/UR1 and yes Y73/Esh virus. In first part this study we demonstrated that UR2, most isolated virus, contains in its genome a unique sequence, ros , nonhomologous src, sequences or transforming acute leukemia viruses. Using cDNAs specific inserts virus genomes, examined existence transcription cellular nucleotide related three new ( fps, ) various cells. The progenitor for these (c- were present uninfected chicken DNA one few copies per haploid genome. These c- detectable wide variety vertebrates, homology between viral was inversely phylogenetic distance animal species. pattern expression different tissues chickens found be each gene. generally repressed many tissues, but expressed at higher levels bone marrow (2.5 cell) lung (1.1 cell), whereas mainly transcribed kidney cell). On other hand, transcripts easily all analyzed high (26 expressions unaffected by infection with contained genes. cultures quail transformed cells derived from tumors induced chemical carcinogens, four remained unaltered, compared normal tissues. Images