Method for quantification of an analyte

作者: Carl T. Prof. Dr. Wittwer , Sabine Dr. Lohmann , Martin Dr. Gutekunst

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摘要: Methods are provided for quantifying the concentration of a nucleic acid in sample. The methods include contacting sample with an amplifying agent, at least one predetermined locus by subjecting to number amplification, generating amplification curve or array, calculating first, second n th order derivative determining maximum, minimum, zero value derivative, and using calculate initial

参考文章(17)
Cheryl Davey, Lawrence T. Malek, Nucleic acid amplification process ,(1988)
Mark L Collins, Bruce Irvine, Diana Tyner, Eric Fine, Crystle Zayati, Chu-an Chang, Thomas Horn, David Ahle, Jill Detmer, Lu-Ping Shen, Janice Kolberg, Steve Bushnell, Mickey S Urdea, David D Ho, A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml Nucleic Acids Research. ,vol. 25, pp. 2979- 2984 ,(1997) , 10.1093/NAR/25.15.2979
Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen, Monitoring hybridization during pcr ,(1997)
Paul M. Lizardi, Hilda M. L. Buyoli, Sanjay Tyagi, Cesar E. Guerra, Fred R. Kramer, Nucleic acid process containing improved molecular switch ,(1988)
Stephen Joel Scharf, Norman Arnheim, Henry Anthony Erlich, Kary Banks Mullis, Glenn Thomas Horn, Randall Keichi Saiki, Process for amplifying, detecting, and/or-cloning nucleic acid sequences ,(1986)
B. Haendler, R. Hofer-Warbinek, E. Hofer, Complementary DNA for human T-cell cyclophilin. The EMBO Journal. ,vol. 6, pp. 947- 950 ,(1987) , 10.1002/J.1460-2075.1987.TB04843.X
Carl T. Wittwer, Kirk M. Ririe, David R. Hillyard, Randy P. Rasmussen, System and methods for monitoring for dna amplification by fluorescence ,(1997)
D. Lassner, H. Remke, A. K. Rost, Th. Kohler, B. Pustowoit, B. Thamm, Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods ,(1995)
C.T. Wittwer, K.M. Ririe, R.V. Andrew, D.A. David, R.A. Gundry, U.J. Balis, The LightCycler: a microvolume multisample fluorimeter with rapid temperature control BioTechniques. ,vol. 22, pp. 176- 181 ,(1997) , 10.2144/97221PF02