Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2.

摘要: Mitogen-induced signal transduction is mediated by a cascade of protein phosphorylation and dephosphorylation. One the immediate responses mitogen stimulation activation family kinases known as mitogen-activated kinase or extracellular signal-regulated (ERK). MEK (MAP ERK kinase) upstream activator ERK. Two cDNAs, MEK1 MEK2, were cloned sequenced. MEK2 encode 393 400 amino acid residues, respectively. The human shares 99% sequence identity with murine 80% MEK2. Both expressed in Escherichia coli shown to be able activate recombinant ERK1 vitro. purified stimulated threonine tyrosine on concomitantly activated activity more than 100-fold. showed lower an compared from tissue. However, can serum-stimulated cell extract MEKs, manner similar ERKs, are likely consist related proteins playing critical roles transduction.