Downregulation of KIF23 suppresses glioma proliferation.

摘要: To identify therapeutic molecular targets for glioma, we performed modified serological identification of antigens by recombinant complementary DNA (cDNA) expression cloning using sera from a mouse glioma model. Two clones, kinesin family member 23 (Kif23) and structural maintenance chromosomes 4 (Smc4), were identified as through immunological reaction with mice harboring synergic GL261 intratumoral inoculation mutant herpes simplex virus. The human Kif23 homolog KIF23 is nuclear protein that localizes to the interzone mitotic spindles, acting plus-end-directed motor enzyme moves antiparallel microtubules in vitro. Expression analysis revealed higher level tissues than normal brain tissue. introduction small interfering RNA (siRNA) targeting into two different cell lines, U87MG SF126, downregulated expression, which significantly suppressed proliferation siRNA-treated cells exhibited larger bodies or more nuclei compared control cells. In vivo xenograft showed siRNA/DNA chimera-treated tumors smaller treated chimera. Taken together, our results indicate downregulation decreases may be novel target malignant glioma.