CpG methylation of promoter region inactivates E-cadherin gene in renal cell carcinoma.
作者： Dana Nojima , Koichi Nakajima , Long-Cheng Li , Joseph Franks , Leopoldo Ribeiro-Filho
DOI: 10.1002/MC.1060
关键词:
摘要: CpG methylation in the promoter region has been shown to be important regulation of genes implicated malignant transformation. The present study was designed test hypothesis that E-cadherin gene may inactivate its expression renal cell carcinoma. To this hypothesis, five kidney cancer lines and 34 microdissected carcinoma samples were analyzed for protein by reverse transcription–polymerase chain reaction immunohistochemistry, respectively. regions sodium bisulfite genome sequencing technique. Our results show all normal tissue expressed protein. Of tissues analyzed, 67% (23 34) lacked expression, with an associated increase methylation, compared tissue. methylated accompanied a loss expression. treatment demethylating agent 5-aza-2′-deoxycytidine restored mRNA lines. This is first report shows inactivation through hypermethylation gene. these experiments contribute understanding role © 2001 Wiley-Liss, Inc.
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