CD44 alternative splicing and hnRNP A1 expression are associated with the metastasis of breast cancer.
作者： TIING JEN LOH , HEEGYUM MOON , SUNGHEE CHO , HANA JANG , YONG CHAO LIU
DOI: 10.3892/OR.2015.4110
关键词:
摘要: Abstract CD44 is a transmembrane receptor for hyaluronic acid. pre-mRNA contains 19 exons, 9 of which are alternatively spliced. Among the spliced variants, v4-7 variant, one of v6 exon-containing isoforms that variable exon 4, 5, 6 and 7, confers metastatic potential to non-metastatic cells. Splicing and function different in breast cancer hnRNP A1 ubiquitously expressed protein with an inhibitory splicing. We showed CD44v6 isoform, includes all v6-containing mRNA isoforms, had highest expression level non-metatatic cells (MCF7) when compared (MDA-MB-231) normal (MCF10A). Furthermore we knockdown regulated splicing differently here isoform completely MDA-MB-231 than MCF7 MCF10A cells, whereas similar pattern isoforms. RT-PCR analysis predominantly c5v6v7v8v9v10c6 isoform. However, addition this also c5v6v8v9v10c6 c5v6c6 found significantly reduced c5v6v8v9v10c6, promoted c5v6c6. induced cell death. In addition, decrease invasion Our results indicate hnRNP A1 has specific on
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