Characterization of Murine Monoclonal Antibodies Reactive to Either the Human Epidermal Growth Factor Receptor or HER2/neu Gene Product
作者： Robert M. Hudziak , Mary Anna Napier , Michael T. Lipari , Brian M. Fendly , Axel Ullrich
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摘要: High levels of expression either the epidermal growth factor receptor or receptor-like HER2/neu gene product p185HER2 have been observed in a variety human malignancies. Because association this high level with certain tumors, we generated panel monoclonal antibodies specific for to study their structure, function, and antigenic domains normal neoplastic states. We used epidermoid carcinoma line A431 generate five which immunoprecipitate receptor. These bind extracellular domain demonstrate variable effects on binding. stably transfected NIH 3T3 cell expressing produce characterize 10 p185HER2. do not cross-react The characteristics potential applications these will be discussed.
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