Tumour control by whole brain irradiation of anti-VEGF-treated mice bearing intracerebral glioma

摘要: Aim of the study: Tumour angiogenesis and invasion are key features glioblastoma multiforme (GBM). Angiogenesis inhibitors increase progression-free survival (PFS) recurrent GBM patients. VEGF inhibition controls bulk tumour growth by angiogenesis, but does not inhibit invasive component. We investigated if can be controlled combining anti-VEGF treatment with irradiation plus surrounding brain in an orthotopic murine model for GBM. Methods materials: cell line U251-NG2 was inoculated through a guide screw right frontal lobe 53 athymic nude mice. Pegaptanib (a slow-releasing aptamer against VEGF) injected bed either or followed implanted I-125 seeds. and/or were compared sham-treated controls, resulting four groups 10-15 mice each. After 6 weeks treatment, histological analysis performed on all brains. Results: locally deposited pegaptanib decreased blood vessel density, increased hypoxia. still allowed formation satellites. Irradiation size suppressed Combined further PFS. directly correlated Concluding statement: The anti-tumour effects local partially circumvented Additional is effective slowing down proliferation these components.