Activation of the Sarcophaga lectin gene promoter by (A + T)-stretch binding protein
作者： Naohiko Aozasa , Hirohisa Shiraishi , Mayumi Nakanishi-Matsui , Ayako Kobayashi , Kazuhisa Sekimizu
DOI: 10.1046/J.1432-1327.2001.02133.X
关键词: Complementary DNA 、 Molecular biology 、 Sarcophaga lectin 、 Transcription (biology) 、 Luciferase reporter 、 Transcription factor 、 Gene 、 Biology 、 Binding protein 、 Promoter 、 Biochemistry
摘要: Previously, we purified and isolated a cDNA for (A + T)-stretch binding protein (ATBP) that binds to (A + T)-stretches in the 5′ upstream region of Sarcophaga lectin gene [Nakanishi-Matsui, M., Kubo, T. & Natori, S. (1995) Eur. J. Biochem.230, 396–400]. Here, used luciferase reporter examine effect ATBP on transcription gene. Deletion experiments revealed activates sequence-dependent manner, at least N-terminal 25 residues, three Zn-finger domains, an acidic domain third hydrophobic are indispensable its function. Furthermore, synergistic was detected between Dif, suggesting is involved activation insect immunity genes.
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