Incidence of chromosomal imbalances in advanced colorectal carcinomas and their metastases.

摘要: Comparative genomic hybridization (CGH) was used to screen 54 advanced colon carcinomas, i.e., 24 primary tumors and 30 metastases, for chromosomal alterations. Using a sensitive statistical method the determination of DNA imbalances histograms analysis incidence changes, we identified over-representation chromosome 20q as most common alteration being present in 100% cases. High deletions were observed on 18q21–18q23 (96%), 4q27–4q28 4p14 (87%), 5q21 (81%), 1p21–1p22 (72%), 21q21 (74%), 6q16 3p12 (66%), 8p24–8p21 9p21 (64%), 11q22 14q13–14q21 (64%). Further frequent found 7q12–7q11.2 (75%), 16p11–16p12 (70%), 19p13 9q34 (67%), 19q13 13q34 13q13 17q21 (59%), 22q11 (61%), 8q24 (57%), 1q21 (57%). Pronounced gains losses defined regions which ratio profiles exceeded values 1.5 0.5, respectively, frequently colocalized with peaks curve. The use difference comparison tumor subgroups well case-by-case histogram 15 paired samples several above alterations relevant progression metastasis formation. study additional loci delineates more precisely those that have been previously reported. For comparative purposes, made our data (ratio profiles, clinicopathological parameters, histograms) available at interactive web site http://amba.charite.de/cgh, where changes can be determined individual parameters applied CGH results.