Gene Expression Profiling of Rat Fetuses Exposed to 2-Dimensional Ultrasound
作者： Zvonko Hocevar , Janez Rozman , Alja Videtic Paska , Robert Frangez , Tomaz Vaupotic
DOI: 10.7863/JUM.2012.31.6.923
关键词:
摘要: OBJECTIVES This study evaluated the possible effects of ultrasound (US) on gene expression in brain tissue rat embryos. METHODS Four groups (n = 5 each) pregnant Wistar Han rats were exposed to US for different durations (55, 100, 145, and 195 seconds) via a multifrequency transducer 2-dimensional imaging mode with pulse duration 1.29 microseconds, repetition frequency 1 kHz, derated spatial-peak pulse-average intensity 222.4 W/cm(2) day 5, 9, 7, or 13 gestation. Gene profiling was performed fetal per group) by quantitative reverse transcription-polymerase chain reaction arrays. RESULTS The results indicated substantial alterations expression. most differentially expressed genes Adamts5, Gadd45a, Npy2r, Chrna1, which are implicated important developmental signaling pathways. CONCLUSIONS On basis our findings, routine short examinations monitoring development not contraindicated, but prolonged exposures should be used only when needed obtain diagnostic information.
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