Selenite cytotoxicity in drug resistant and nonresistant human ovarian tumor cells.
作者： Paula B. Caffrey , Gerald D. Frenkel
DOI:
关键词:
摘要: Our previous studies on selenite cytotoxicity led us to hypothesize that drug resistant tumor cells with high intracellular glutathione will exhibit a degree of sensitivity selenite. To examine this we studied the effects human ovarian (NIH:OVCAR-3) in three assays cytotoxicity: proliferation; cell viability (trypan blue exclusion); and attachment solid matrix. The were sensitive low levels selenite: concentrations as 5 microM inhibited proliferation attachment; was decreased by 20 microM. In each these NIH:OVCAR-3 more than (A2780) cells. These results suggest potential for utilization treatment some tumors.
aacrjournals.org参考文章(28)
Leendert N. Vernie, Martha De Vries, Leendert Karreman, Randolf J. Topp, Willy S. Bont, Inhibition of amino acid incorporation in a cell-free system and inhibition of protein synthesis in cultured cells by reaction products of selenite and thiols Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. ,vol. 739, pp. 1- 7 ,(1983) , 10.1016/0167-4781(83)90037-4
A. Rogan, T. Hamilton, R. Young, R. Klecker, R. Ozols, Reversal of adriamycin resistance by verapamil in human ovarian cancer Science. ,vol. 224, pp. 994- 996 ,(1984) , 10.1126/SCIENCE.6372095
L.N. Vernie, C.J. Homburg, W.S. Bont, Inhibition of the growth of malignant mouse lymphoid cells by selenodiglutathione and selenodicysteine Cancer Letters. ,vol. 14, pp. 303- 308 ,(1981) , 10.1016/0304-3835(81)90159-2
L. J. Goldstein, H. Galski, A. Fojo, M. Willingham, S.-L. Lai, A. Gazdar, R. Pirker, A. Green, W. Crist, G. M. Brodeur, M. Lieber, J. Cossman, M. M. Gottesman, I. Pastan, Expression of Multidrug Resistance Gene in Human Cancers Journal of the National Cancer Institute. ,vol. 81, pp. 116- 124 ,(1989) , 10.1093/JNCI/81.2.116
A. T. Fojo, K. Ueda, D. J. Slamon, D. G. Poplack, M. M. Gottesman, I. Pastan, Expression of a multidrug-resistance gene in human tumors and tissues Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 265- 269 ,(1987) , 10.1073/PNAS.84.1.265
Garth L. Nicolson, Cancer metastasis: tumor cell and host organ properties important in metastasis to specific secondary sites. Biochimica et Biophysica Acta. ,vol. 948, pp. 175- 224 ,(1988) , 10.1016/0304-419X(88)90010-8
Paula B. Caffrey, Gerald D. Frenkel, Selenite-induced inhibition of colony formation by buthionine sulfoximine-sensitive and resistant cell lines Biological Trace Element Research. ,vol. 35, pp. 129- 136 ,(1992) , 10.1007/BF02783725
Louise K. Hosking, Richard D.H. Whelan, Sharon A. Shellard, Philip Bedford, Bridget T. Hill, An evaluation of the role of glutathione and its associated enzymes in the expression of differential sensitivities to antitumour agents shown by a range of human tumour cell lines. Biochemical Pharmacology. ,vol. 40, pp. 1833- 1842 ,(1990) , 10.1016/0006-2952(90)90364-Q
Daniel Medina, Carol J. Oborn, Differential effects of selenium on the growth of mouse mammary cells in vitro Cancer Letters. ,vol. 13, pp. 333- 344 ,(1981) , 10.1016/0304-3835(81)90062-8
Robert F. Ozols, Karen G. Louie, Jacqueline Plowman, Brent C. Behrens, Robert L. Fine, Donald Dykes, Thomas C. Hamilton, Enhanced melphalan cytotoxicity in human ovarian cancer in vitro and in tumor-bearing nude mice by buthionine sulfoximine depletion of glutathione. Biochemical Pharmacology. ,vol. 36, pp. 147- 153 ,(1987) , 10.1016/0006-2952(87)90392-3