Multipartite Regulation of rctB, the Replication Initiator Gene of Vibrio cholerae Chromosome II

摘要: Replication initiator proteins in bacteria not only allow DNA replication but also often regulate the rate of initiation as well. The regulation is mediated by limiting synthesis or availability proteins. applicability this principle demonstrated here for RctB, smaller two chromosomes Vibrio cholerae. A strong promoter rctB gene named rctBp was identified and found to be autoregulated Escherichia coli. Promoter activity lower V. cholerae than E. coli, a part reduction likely due autorepression. Sequences upstream rctBp, implicated earlier control, enhanced repression. action sequences required that they present cis, implying long-range interactions control activity. second specific chromosome II replication, rctA, reduced translation, most antisense RNA control. Finally, optimal dependent on Dam. Increasing RctB trans increased copy number miniplasmid carrying oriCIIVC, can replication. multiple modes are expected reduce fluctuations concentration thereby help maintain homeostasis.