Surface functionalization of polyurethane for the immobilization of bioactive moieties on tissue scaffolds.

摘要: Segmented polyurethanes are widely used in medical devices because of their desirable physical and chemical properties proven biocompatibility. While polyurethane is many respects an ideal tissue scaffold, its performance no better than other synthetic polymers, which due part to surface properties. Here, we describe a method for the functionalization scaffolds that involves physically incorporating another polymer (poly(ethyleneimine)) such integrity bulk retained; primary amine groups thus incorporated into enable subsequent coupling with dextran recombinant peptides by means reductive amination. The efficacy grade aliphatic poly(ether)urethane verified analysis (secondary ion mass spectrometry) fluorescence spectrophotometric assays adapted specifically this purpose. Further assessment surfaces direct cell contact cellular response terms coverage morphology before after modification specific peptide sequences GRGDSPK Fibrillin-1 fragment PF9.