Cholangiocarcinoma: Correlation between Molecular Profiling and Imaging Phenotypes

摘要: Purpose To investigate associations between imaging features of cholangiocarcinoma by visual assessment and texture analysis, which quantifies heterogeneity in tumor enhancement patterns, with molecular profiles based on hypoxia markers. Methods The institutional review board approved this HIPAA-compliant retrospective study CT images intrahepatic cholangiocarcinoma, obtained before surgery. Immunostaining for markers (EGFR, VEGF, CD24, P53, MDM2, MRP-1, HIF-1α, CA-IX, GLUT1) was performed pre-treatment liver biopsies. Quantitative phenotypes were determined analysis gray level co-occurrence matrixes. The correlations quantitative phenotypes, qualitative (measured radiographic inspection alone), expression levels the from 25 tumors assessed. Results Twenty-five patients included a median age 62 years (range: 54–84). size 10.2 cm 4–14), 10 (40%) single tumors, 90% moderately differentiated. Positive immunostaining recorded VEGF 67% cases, EGFR 75%, CD24 55%. On multiple linear regression correlated significantly (R2 = 0.4, p<0.05 R2 0.2, p<0.05, respectively), while trend demonstrated 0.33, p 0.1). Three (P<0.05), however, none phenotypes. Conclusion Quantitative as defined specific hypoxia, regardless conventional features.