Up-Regulation of the α2,6-Sialyltransferase Messenger Ribonucleic Acid Increases Glycoconjugates Containing α2,6-Linked Sialic Acid Residues in Granulosa Cells during Follicular Atresia of Porcine Ovaries

摘要: The sugar chains in cellular glycoconjugates have many biological functions. Extensive morphological development and remodeling occur the ovary of female animals. This caused us to study glycobiological characteristics ovarian cells, particularly granulosa cells that undergo apoptosis during follicular atresia. lectin Sambucus sieboldiana agglutinin (SSA) specific for Siaalpha2,6Gal/GalNAc showed positive staining only atretic follicles porcine ovaries by histochemistry. Lectin blot analysis SSA demonstrated glycoproteins follicles. Furthermore, we performed backbone structures SSA-positive glycans carried cell increased atresia glycosidase treatment. Most these were Siaalpha2,6Galbeta1,4GlcNAc on complex-type N-glycans, suggesting ST6Gal I four distinct alpha2,6-sialyltransferases catalyzes alpha2,6-sialic acid transfer most Reverse transcription-polymerase chain reaction expression mRNA was up-regulated These results suggested alteration is involved some processes apoptosis.