Evaluation of a modified EC Four Plate Method to detect antimicrobial drugs.
作者： Deborah Currie , Lorraine Lynas , D. Glenn Kennedy , W. John McCaughey
DOI: 10.1080/02652039809374694
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摘要: This paper describes a modification of the EC Four Plate Method based on microbial growth inhibition Bacillus subtilis agar medium at pH 6.0, 7.2 and 8.0 Micrococcus luteus developed to cope with large numbers samples. The method's performance was evaluated by determining Minimum Inhibitory Concentrations 66 commonly used drugs between‐assay variation antimicrobial control standards. modified method proved particularly sensitive for β‐lactams, tetracy‐clines, quinolones, marcrolides lincosamides least anticoccidials nitrofurans. 6.0 plates were more 39 antimicrobials (59%) whereas two (B. subtilis, M. luteus) most 27 (41%). Muscle samples taken from 1830 routine meat inspection investigations between 1994 1996. Of 38 (2%) positive carcasses, following confirmed above MRL: penicillin G (10)...
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