The p53 R72P polymorphism does not influence cervical cancer development in a Portuguese population: a study in exfoliated cervical cells.

摘要: The interaction between the E6 protein of high-risk human papillomaviruses (HPVs) with p53 seems to be crucial in cervical carcinogenesis. presence Arg/Arg genotype at codon 72 TP53 gene was characterized as a risk factor development cancer. However, role this polymorphism remains controversial and some authors suggested that origin DNA (blood or exfoliated cells) might influence these results. This study analyzed effect (R72P) cells women from northern region Portugal using two methodologies: allele-specific polymerase chain reaction real-time reaction. We studied 700 which showed: 334 cases without cancer lesion (N), 114 low-grade squamous intraepithelial lesions (LSIL), 107 high-grade (HSIL), 20 invasive cancers (ICC) 125 atypical unknown significance (ASCUS). No statistically significant differences controls were found, regarding R72P cytological classification, high risk-HPV infection HPV16 (P = 0.336, P = 0.945, P = 0.964, respectively). Also, median age onset for LSIL, HSIL, ICC not (P = 0.674, P = 0.810, P = 0.928, Therefore, hypothesis have an increased developing failed proven study. Moreover, our reveals results are reliable compared studies on blood. J. Med. Virol. 80:424–429, 2008. © 2008 Wiley-Liss, Inc.