Environmental and occupational biomonitoring using the Comet assay.

摘要: Biomonitoring of human populations exposed to potential mutagens or carcinogens can provide an early detection system for the initiation cell disregulation in development cancer. In recent years, Comet assay, also known as a "single gel" (SCG) electrophoresis has become important tool assessing DNA damage populations. This is method choice population-based studies environmental and occupational exposure air pollutants, metals, pesticides, radiation, other xenobiotics we show this review. To appreciate role assay field biomonitoring, review data from 122 that employed assay. These evaluated versus exposures levels cells individuals each case. Our literature reveals importance need establish standard methodological conditions affect unwinding times tail values (tail length, DNA, moment), with goal being able compare collected different laboratories throughout world. The susceptible subtle artifacts manipulation depending on type timing sampling performed. Therefore, reporting detected by context how was created needs be reported considered interpretation results. success reflected its use over past 20 years increasing number continue report use. As shortcomings are identified detection, will improved reliability biomarker biomonitoring studies.