Comparative genetic patterns of glioblastoma multiforme: Potential diagnostic tool for tumor classification

摘要: Cytogenetic and molecular genetic studies of glioblastoma multiforme (GBM) have shown that the most frequent alterations are gains chromosome 7, losses 9p loci 10, gene amplie cation, primarily epidermal growth factor receptor ( EGFR) gene. Although this proe le is potentially useful in distinguishing GBM from other tumor types, techniques used tend to be labor intensive, some can detect only or loci. Comparative genomic hybridization (CGH) a powerful technique capable identifying both DNA sequences. The present study compares CGH evaluation 22 with classic cytogenetics, loss heterozygosity by allelotyping, cation Southern blot analysis determine reliability characterization GBM. karyotypic data were consistent showing gain 7 accompanied 10 as abnormality, followed 17 cases. Loss chromosomes (19/22) (9/22) cone rmed underrepresentation CGH. Genomic cations observed 5 cases where was detected analysis. show equally reliable, compared more established methods, for recognizing prominent associated support its use plausible adjunct glioma classie cation. Neuro-Oncology 2, 164-173, 2000