Mitogen-activated protein kinase, ERK1/2, is essential for the induction of vascular endothelial growth factor by ionizing radiation mediated by activator protein-1 in human glioblastoma cells.
作者： Katsuharu Mori , Masaharu Tani , Kensaku Kamata , Hideki Kawamura , Yoshishige Urata
DOI: 10.1080/10715760000300711
关键词:
摘要: Vascular Endothelial Growth Factor (VEGF)/Vascular Permeability plays an important role in angiogenesis and cell proliferation of cancer cells. Glioblastoma cells are most malignant show resistance to radiation therapy inducing VEGF cause brain edema. In the present study, regulatory mechanism expression by ionizing was studied three human glioblastoma Induction mRNA dependent on dose incubation time. Activator protein-1 (AP-1) activated 10 Gy 1 h T98G electrophoretic mobility shift assay. We constructed chimeric genes containing various regions promoter gene coding region for chloramphenicol acetyltransferase (CAT) transiently transfected them CAT assay with AP-1 site demonstrated that activity enhanced radiation. Immun...
informahealthcare.com参考文章(29)
Nadia Rosenthal, [72] Identification of regulatory elements of cloned genes with functional assays Methods in Enzymology. ,vol. 152, pp. 704- 720 ,(1987) , 10.1016/0076-6879(87)52075-4
Adapa V. Prasad, Natarajan Mohan, Bysani Chandrasekar, Martin L. Meltz, Activation of Nuclear Factor κB in Human Lymphoblastoid Cells by Low-Dose Ionizing Radiation Radiation Research. ,vol. 138, pp. 367- 372 ,(1994) , 10.2307/3578685
Georg Breier, Marcia Machein, Annette Damert, Masaki Q. Fujita, Masaki Q. Fujita, Werner Risau, Karl H. Plate, Douglas Hanahan, Up-Regulation of Vascular Endothelial Growth Factor Expression in a Rat Glioma Is Conferred by Two Distinct Hypoxia-driven Mechanisms Cancer Research. ,vol. 57, pp. 3860- 3864 ,(1997)
Shizuo Shirai, Shingo Takano, Hideo Suzuki, Shinichi Kondo, Tooru Maruno, Yoshihiko Yoshii, Tadao Nose, Concentration of Vascular Endothelial Growth Factor in the Serum and Tumor Tissue of Brain Tumor Patients Cancer Research. ,vol. 56, pp. 2185- 2190 ,(1996)
E. Tischer, R. Mitchell, T. Hartman, M. Silva, D. Gospodarowicz, J.C. Fiddes, J.A. Abraham, The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. Journal of Biological Chemistry. ,vol. 266, pp. 11947- 11954 ,(1991) , 10.1016/S0021-9258(18)99049-6
B Teale, S Singh, K.K. Khanna, D Findik, M.F. Lavin, Purification and characterization of a DNA-binding protein activated by ionizing radiation. Journal of Biological Chemistry. ,vol. 267, pp. 10295- 10301 ,(1992) , 10.1016/S0021-9258(19)50017-5
Ken Samoto, Tadahisa Shono, Mayumi Ono, Michihiko Kuwano, Masashi Fukui, Kiyonobu Ikezaki, Kimitoshi Kohno, Expression of vascular endothelial growth factor and its possible relation with neovascularization in human brain tumors. Cancer Research. ,vol. 55, pp. 1189- 1193 ,(1995)
Natarajan Mohan, Martin L. Meltz, Induction of nuclear factor κB after low-dose ionizing radiation involves a reactive oxygen intermediate signaling pathway Radiation Research. ,vol. 140, pp. 97- 104 ,(1994) , 10.2307/3578574
Amato J. Giaccia, Albert C. Koong, Eunice Y. Chen, Hypoxia causes the activation of nuclear factor kappa B through the phosphorylation of I kappa B alpha on tyrosine residues. Cancer Research. ,vol. 54, pp. 1425- 1430 ,(1994)
D. Gospodarowicz, J. A. Abraham, J. Schilling, Isolation and characterization of a vascular endothelial cell mitogen produced by pituitary-derived folliculo stellate cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 7311- 7315 ,(1989) , 10.1073/PNAS.86.19.7311